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Natural Killer (NK) Cell Activating Factor Produced by a Human T‐Cell Hybridoma
Author(s) -
Kobayashi Yoshiro,
Yoshimatsu Kentaro,
Osawa Toshiaki
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01620.x
Subject(s) - biology , concanavalin a , microbiology and biotechnology , interleukin 2 , natural killer cell , cell culture , k562 cells , lymphokine , cell , immunology , cytotoxicity , cytokine , biochemistry , immune system , in vitro , genetics
A human T‐cell hybridoma (KC8–1.10), whose culture supernatant augments peripheral blood lymphocytes (PBL)‐mediated spontaneous cytotoxicity against K562 cells, was established. This activity [natural killer (NK) cell activating activity] appears to be not due to interferon‐γ (IFN‐γ) and interleukin‐2 (IL‐2) for the following reasons: 1) KC8–1.10 produced negligible or small amounts of IFNs and IL‐2. 2) The NK cell activating activity in the KC8–1.10 culture supernatant was not neutralized by anti‐IFN‐γ antiserum and stable even after pH 2 treatment for 24 hr, which is known to destroy IFN‐γ activity, 3) IL‐2‐dependent cell line absorbed IL‐2 more efficiently than it absorbed the NK cell activating activity, and the latter activity was not retained by Blue Sepharose column in contrast with IL‐2. The NK cell activating factor in the KC8–1.10 culture supernatant appears to be a glycoprotein, because the activity was abolished with pronase treatment or with boiling for 5 min and because the activity was retained by concanavalin A‐ and Pisum sativum agglutinin‐agarose. Finally it was found that the NK cell activating activity requires Leu 11b + cells to exert its effect.