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New Enzyme Immunoassays for Specific Assay and General Detection of Trypanosoma cruzi , Epimastigotes
Author(s) -
Kitagawa Tsunehiro,
Iwamoto Masayuki,
Zhao Liping,
Kanbara Hiroji
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01616.x
Subject(s) - trypanosoma cruzi , amastigote , immunoassay , biology , antibody , chagas disease , antiserum , microbiology and biotechnology , virology , leishmania , parasite hosting , immunology , computer science , world wide web
A highly specific competitive enzyme‐linked immunosorbent assay for the epimastigote of Tulahuen strain was developed by using the usual 3 immunological reagents, a rabbit antiserum specific for T. cruzi , epimastigote of Tulahuen strain, β‐D‐galactosidase‐labeled goat anti‐rabbit immunoglobulin G and the solid‐phase cell fragments of the epimastigote of Tulahuen strain. A new method, the selected antibody enzyme immunoassay (SAEIA) which generally detected all strains of the epimastigote tested with the same working range, was developed by changing only the solid‐phase antigen to the epimastigote of Y strain among the 3 immunological reagents. Both assays permitted us to measure accurately as little as 1,000 parasites per assay tube. Scope of the SAEIA was limited to the epimastigote. Both life‐cycle forms of T. cruzi which appear in mammals, amastigote and trypomastigote, and other kinetoplastids showed low cross‐reaction values by the assay. The assay principle of the new method and a preliminary study to apply the SAEIA for finding the field T. cruzi ‐infected insect vectors were also reported.