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Suppression of Lymphocyte Signal Transduction by Murine Mastocytoma Ascites
Author(s) -
Ding Linna,
Isobe Kenichi,
Yoshida Tomoaki,
Nakashima Izumi
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01609.x
Subject(s) - mastocytoma , biology , ascites , concanavalin a , lymphocyte , cytotoxic t cell , ctl* , neoplasm , immune system , signal transduction , spleen , antigen , t lymphocyte , immunology , microbiology and biotechnology , in vitro , medicine , cd8 , biochemistry , genetics
The lymphocyte signal transduction, as determined by intracellular free Ca 2+ mobilization of concanavalin A‐stimulated T lymphocytes and of antiimmunoglobulin μ chain antibody‐stimulated B lymphocytes, was suppressed in spleen cells from mice injected with murine Pl.HTR mastocytoma‐induced ascites and in spleen cells treated with the ascites in vitro. The suppression was observed both at the peak level and in the reactive pattern of Ca 2+ influx. In the suppression, the ascites were replaceable with tumor culture supernatants or tumor homogenates. Correspondingly, primary and secondary cytotoxic T lymphocyte (CTL) responses of DBA/2 mice to allogeneic antigen were also significantly suppressed by injection of the syngeneic Pl.HTR tumor‐derived ascites. This new finding suggested that the mechanism of the tumorous ascites or of the tumor‐derived factor‐mediated immunosuppression involves at least in part the suppression of the early event of the signal transduction for lymphocyte activation.