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Sensitive Detection of Viral Antigens with a New Method, “Laser Magnet Immunoassay”
Author(s) -
Mizutani Hiroko,
Suzuki Miwako,
Mizutani Hiromichi,
Fujiwara Koichi,
Shibata Shuichi,
Arishima Koichi,
Hoshino Mitsutoshi,
Ushijima Hiroshi,
Honma Hitoshi,
Kitamura Takashi
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01605.x
Subject(s) - immunoassay , antigen , virology , biology , magnetite , virus , antibody , laser , materials science , immunology , optics , physics , paleontology
A new method, “laser magnet immunoassay” (LMIA), has been developed for sensitive detection of viral antigens. Target viruses captured on microbeads were made to react with antibodies labeled with magnetite particles. In a magnetic field, magnetically labeled antigens dispersed in water were attracted to and concentrated at one point on the surface, resulting in the lifting up of a small surface area. A laser beam which was incident on the point reflected, making an interference fringe. The intensity of the fringe indicates the amount of the magnetite conjugated with antigen. A very low concentration of antigens, such as 5 particles of influenza virus and 0.1 pg/ml of human immunodeficiency virus (HIV) p24 antigen in human serum, could be detected by this method. Application of this method to diagnoses of viral diseases in early stages is discussed.