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Electron Microscopic Studies on the in vitro Proliferation of Spotted Fever Group Rickettsia Isolated in Japan
Author(s) -
Amano Kenichi,
Hatakeyama Hiroshi,
Sasaki Yoshimitsu,
Ito Reietsu,
Tamura Akira,
Suto Tsunehisa
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01594.x
Subject(s) - spotted fever , rickettsia , biology , rickettsiaceae , rickettsiosis , in vitro , electron microscope , rickettsiales , virology , microbiology and biotechnology , bacteria , virus , genetics , physics , optics
Rickettsia was isolated from a patient with Japanese spotted fever, and its proliferation in cultured green monkey kidney cells was observed by electron microscopy. In the course of this study, we observed fusion of infected cells to uninfected cells which may be a way of spreading the rickettsiae from a cell to another. On the other hand, whirlpool‐like, multilayer membranous structures, similar to the mesosomes of gram‐negative bacteria, were sometimes seen in the rickettsial cells. The other profiles common to the other rickettsiae in spotted fever group were observed, such as the electron‐lucent halo zone around the rickettsiae, and external fibrous materials on their surface, but intranuclear multiplication was rarely observed.