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Expression of Clumping and Fibrinogen‐Binding Activities of Staphylococcus aureus at Various Growth Stages
Author(s) -
Yonemasu Kunio,
Sasaki Takako,
Ohmae Riichi,
Kashiba Syuzo
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01571.x
Subject(s) - fibrinogen , staphylococcus aureus , biology , microbiology and biotechnology , micrococcaceae , staphylococcus , biochemistry , bacteria , genetics
Clumping and fibrinogen‐binding activities of 4 Staphylococcus aureus strains (Cowan I, Newman D2C, Wood 46 and NCTC 5655) were assayed with a semiquantitative clumping test and an enzyme‐linked immunosorbent assay (ELISA), respectively. Distinct positive clumping was detected with whole cells of the 3 strains except Wood 46. Amounts of fibrinogen required for a definite clumping depended greatly on strains as well as on their growth phases. On the other hand, fibrinogen‐binding activities were detected both in culture supernatants and in cell lysates of all the 4 strains, and the levels were rather comparable with one another and relatively steady through their growth cycles. No significant correlation was thus found among expression behavior of clumping and fibrinogen‐binding activities.