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Formation of Vesiculated Large Bodies of Staphylococcus aureus L‐Form in a Liquid Medium
Author(s) -
Yabu Kunihiko
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01570.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , biology , liquid medium , bacteria , chromatography , chemistry , genetics
A method is described in which cells of Staphylococcus aureus can be converted to vesiculated large bodies of L‐form. When coccal cells were incubated in a liquid growth medium containing D‐cycloserine, N ‐acetylmuramidase and subtilisin, a large number of vesiculated large bodies were formed. Electron microscopy revealed that development of internal vesicles arose after 6 hr of incubation. When growth inhibitory concentrations of rifampicin, novobiocin, or chloramphenicol were added to the culture at 6 hr of incubation, small‐sized nonvesiculated bodies were produced instead of vesiculated forms. The viability of cultures was reduced by rifampicin and novobiocin but not by chloramphenicol.