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Rapid Method for Isolation and Staining of Bacterial Lipopolysaccharide
Author(s) -
AlHendy Ayman,
Toivanen Paavo,
Skurnik Mikael
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01562.x
Subject(s) - staining , isolation (microbiology) , lipopolysaccharide , biology , silver stain , microbiology and biotechnology , bacteria , chromatography , gram staining , chemistry , immunology , antibiotics , genetics
Classically bacterial lipopolysaccharide (LPS) purification and silver staining take several days. We designed a simple and fast method for LPS isolation which when combined with silver staining using Pharmacia PhastSystem™ both can be completed in few hours. The purity of LPS isolated by this simple method may not be comparable to that by the phenol‐water method hence we recommend this rapid isolation and staining procedures for simple and fast study of LPS patterns in gels.