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Sensitivity of Bacteria to NaNO 2 and to L‐Arginine‐Dependent System in Murine Macrophages
Author(s) -
Saito Shinji,
Onozuka Kazuyasu,
Shinomiya Hiroto,
Nakano Masayasu
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01561.x
Subject(s) - phagolysosome , bacteria , microbiology and biotechnology , salmonella , arginine , biology , effector , pseudomonas aeruginosa , staphylococcus epidermidis , enterobacteriaceae , phagocytosis , escherichia coli , biochemistry , phagosome , staphylococcus aureus , amino acid , genetics , gene
Sensitivity of bacteria to NO 2 ‐/NO and to L‐arginine‐dependent system in murine macrophages was tested. The growth of all bacteria tested, Salmonella typhimurium, Pseudomonas aeruginosa and Staphylococcus epidermidis , was significantly inhibited by NaNO 2 at the concentration of 5 to 10 mM. However, L‐arginine‐dependent effector mechanism of LPS‐activated murine macrophages was ineffective in killing these bacteria. It seems that the concentration of NO 2 ‐/NO in phagolysosome in the activated macrophages is insufficient to kill the bacteria in situ. We concluded that L‐arginine‐dependent effector mechanism can hardly work on bacteria killing in activated macrophages.