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The Analysis of the Defense Mechanism against Indigenous Bacterial Translocation in X‐Irradiated Mice
Author(s) -
Kobayashi Toshiya,
Ohmori Toshihiro,
Yanai Minoru,
Kawanishi Gosei,
Mitsuyama Masao,
Nomoto Kikuo
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01560.x
Subject(s) - mesenteric lymph nodes , biology , chromosomal translocation , bacteria , escherichia coli , in vivo , microbiology and biotechnology , lymphatic system , lymph , immunology , spleen , pathology , biochemistry , medicine , genetics , gene
The defense mechanism against indigenous bacterial translocation was studied using a model of endogenous infection in X‐irradiated mice. All mice irradiated with 9 Gy died from day 8 to day 15 after irradiation. The death of mice was observed in parallel with the appearance of bacteria from day 7 in various organs, and the causative agent was identified to be Escherichia coli , an indigenous bacterium translocating from the intestine. Decrease in the number of blood leukocytes, peritoneal cells and lymphocytes in Peyer's patches or mesenteric lymph nodes was observed as early as 1 day after irradiation with 6 or 9 Gy. The mitogenic response of lymphocytes from various lymphoid tissues was severely affected as well. The impairment of these parameters for host defense reached the peak 3 days after irradiation and there was no recovery. However, in vivo bactericidal activity of Kupffer cells in mice irradiated with 9 Gy was maintained in a normal level for a longer period. It was suggested that Kupffer cells play an important role in the defense against indigenous bacteria translocating from the intestine in mice.