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Analysis of the tdh Gene Cloned from a tdh Gene‐ and trh Gene‐Positive Strain of Vibrio parahaemolyticus
Author(s) -
Baba Kiyoshi,
Shirai Hiromasa,
Terai Akito,
Takeda Yoshifumi,
Nishibuchi Mitsuaki
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01554.x
Subject(s) - biology , gene , vibrio parahaemolyticus , genetics , gene duplication , microbiology and biotechnology , nucleic acid sequence , gene cluster , inverted repeat , pair rule gene , direct repeat , gene expression , regulator gene , genome , bacteria
A variant of the gene ( tdh) encoding thermostable direct hemolysin (TDH) was cloned from the chromosome of Vibrio parahaemolyticus AQ3860, which gave positive results in the hybridization tests with the tdh gene probe and the trh ( tdh ‐related hemolysin) gene probe and showed a low level of reaction in an enzyme‐linked immunosorbent assay for TDH. Nucleotide sequence analysis of the cloned gene (tdh5) provided no evidence that tdh5 is evolutionally closer to the trh gene than the other tdh genes. The tdh5 gene was flanked by 40 base‐pair sequences constituting perfect inverted repeats, which may suggest association of the tdh5 gene with insertion sequence‐like structure. These results suggest that the tdh5 gene and the trh gene were not originally produced by gene duplication in AQ3860 but rather that one of the two genes moved into AQ3860 from an external source.