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Detection of Bacillus cereus Flagellar Antigen by Enzyme‐Linked Immunosorbent Assay (ELISA)
Author(s) -
Murakami Tomonori,
Hiraoka Kenji,
Mikami Takeshi,
Matsumoto Tatsuji,
Katagiri Susumu,
Suzuki Masuko
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01551.x
Subject(s) - bacillus cereus , immunogold labelling , antigen , biology , serotype , agglutination (biology) , microbiology and biotechnology , serology , cereus , antibody , latex fixation test , enzyme , virology , bacteria , biochemistry , immunology , genetics
A serological typing scheme of Bacillus cereus has been developed by immunochemical analyses of flagellar antigen using an agglutination method. Enzyme‐linked immunosorbent assay (ELISA) for the classification of flagellar serotype of Bacillus cereus had greater sensitivity, 10–500 times, than that of agglutination method. The specificity of flagellar antigen and antibody was determined by immunogold electron microscopy and ELISA inhibition assay. Application of ELISA is useful for the detection of the small amounts and many kinds of antigen‐antibody reactions.