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Production of H 2 O 2 by Alveolar Macrophages in Experimental Allergic Alveolitis
Author(s) -
Tremblay Guy,
Thibault Sylvie,
Cormier Yvon
Publication year - 1991
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1991.tb01542.x
Subject(s) - zymosan , bronchoalveolar lavage , extrinsic allergic alveolitis , immunology , guinea pig , opsonin , pulmonary alveolus , biology , microbiology and biotechnology , medicine , respiratory disease , phagocytosis , endocrinology , lung , in vitro , biochemistry
Experimental extrinsic allergic alveolitis (EAA) was induced in guinea pigs with Saccharopolyspora rectivirgula. Bronchoalveolar lavages were performed before inducing EAA (day 1, BAL 1), on day 23 (BAL 2), and on day 48 (BAL 3). The number of cells/ml in lavage fluid was increased at BAL 2 (4.79 × 10 6 ) and BAL 3 (4.29 × 10 6 ) compared with BAL 1 (0.56 × 10 6 ). The number of major cell types increased simultaneously, neutrophil becoming the predominant cell type over alveolar macrophages (AM). The production of H 2 O 2 by AM was measured at the different phases of EAA. Adherent AM were either non‐stimulated or triggered with phorbol myristate acetate (PMA), zymosan, S. rectivirgula opsonized with normal guinea pig serum (SRNS), or S. rectivirgula opsonized with guinea pig anti‐S. rectivirgula serum (SRAS). Stimulated AM produced larger quantities of H 2 O 2 than unstimulated cells, PMA being the most potent stimulus. At day 1, AM stimulated with S. rectivirgula and zymosan produced similar quantities of H 2 O 2 . After the induction of the disease, AM stimulated with S. rectivirgula produced larger quantities of H 2 O 2 than with zymosan. Production of H 2 O 2 by AM stimulated with S. rectivirgula or PMA, respectively, stayed the same at day 1 and 23, but increased sharply for both stimuli at day 48. There was no difference between H 2 O 2 production by AM triggered with SRNS or with SRAS. This study shows that: 1) H 2 O 2 production is enhanced, especially by the offending agent, in the course of the disease; 2) the enhanced production of H 2 O 2 by AM is preceded by the development of alveolitis; and, 3) the opsonization with specific antibodies does not enhance the avidity of AM for the antigen.