Premium
Induction of Interferons (IFNs) and Tumor Necrosis Factor (TNF) in Mice by a Novel Glycolipid Trehalose 2,3,6′‐Trimycolate from Rhodococcus aurantiacus (Gordona aurantiaca)
Author(s) -
Fujita Takako,
Sugimoto Nobuko,
Yokoi Fusa,
Ohtsubo Yoshikazu,
Ikutoh Masatoshi,
Kato Yoshiko,
Natsuhara Yayoi,
Oka Shiro,
Yano Ikuya
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb03169.x
Subject(s) - glycolipid , biology , tumor necrosis factor alpha , trehalose , rhodococcus , microbiology and biotechnology , biochemistry , immunology , enzyme
The immunomodifying activity of a novel mycoloyl glycolipid, trehalose 2,3,6′‐trimycolate (GaGM), from a unique psychrophilic acid‐fast bacterium, Rhodococcus aurantiacus , was examined. ICR mice were primed intravenously (i.v.) or intraperitoneally (i.p.) with liposomes containing GaGM (300 μg/mouse), and were administered LPS dissolved in saline (25 μg/mouse, i.v.) 2 weeks later. Two hours after injection of LPS, interferons (IFNs) and tumor necrosis factor (TNF) were induced significantly in mice sera. The increase in activities of IFNs and TNF was approximately paralleled with granuloma formation in spleen of mice primed with GaGM. However, IFNs and TNF were not induced either in mice primed with GaGM but not elicited with LPS, or in those primed with GaGM and elicited by GaGM. Both activities induced were lower in mice primed with trehalose mono‐ or dimycolate from R. aurantiacus (GaTMM, GaTDM) or TDM from Nocardia rubra than in GaGM‐primed mice. Time course study showed that the maximum activity of each interferon (α, β, or γ) was observed at different stages after LPS administration; IFN‐α, IFN‐β, and IFN‐γ appeared 3, 2, and 6 hours most abundantly after LPS administration, respectively.