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Elevated Expression of Proto‐Oncogenes during Interleukin‐5‐Induced Growth and Differentiation of Murine B Lineage Cells
Author(s) -
Migita Masahiro,
Yamaguchi Naoto,
Katoh Shigeki,
Mita Seiji,
Matsumoto Ryoji,
Sonoda Eiichiro,
Tsuchiya Hiroyuki,
Matsuda Ichiro,
Tominaga Akira,
Takatsu Kiyoshi
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01072.x
Subject(s) - biology , haematopoiesis , lymphokine , microbiology and biotechnology , cellular differentiation , tetradecanoylphorbol acetate , cell culture , interleukin 4 , messenger rna , gene expression , cell growth , interleukin 3 , phorbol , secretion , immunology , t cell , cytokine , signal transduction , protein kinase c , endocrinology , il 2 receptor , gene , stem cell , antigen , immune system , biochemistry , genetics
Intcrleukin 5 (IL‐5), a lymphokine produced by helper T cells, is involved in the regulation of growth and differentiation of B cells and other hematopoietic cells. To elucidate IL‐5‐mediated intracellular mechanisms, we have established IL‐5‐dependent and ‐independent murine early B cell lines, J6 and MJ88‐1, respectively, and examined the effect of IL‐5 on the expression of proto‐oncogenes during proliferation. Two‐ to 3.5‐fold increases in the levels of c‐myb, c‐myc, c‐fos , and c‐fms mRNA were observed in J6 cells, compared with those in MJ88‐1 cells. Further, a role of IL‐5 in the proto‐oncogene expression during differentiation was examined by using thymidine‐treated murine B‐cell chronic leukemia BCL 1 ‐B20 cells with growth arrest. After 4‐day culture, the amount of IgM secreted from BCL 1 ‐B20 cells was augmented 4‐6 fold in the presence of IL‐5. Although expression of c‐myb, c‐fos , and c‐fms mRNA did not change, only c‐myc mRNA expression was elevated within 30 min of stimulation with IL‐5 and reached a maximal level by 1 hr. Addition of phorbol 12‐myristate 13‐acetate (PMA) or IL‐4 to the culture of BCL 1 ‐B20 cells inhibited both the IL‐5‐mediated augmentation of IgM secretion and the elevated expression of c‐myc mRNA. These findings suggest that the IL‐5 signal may be associated with the up‐regulation of c‐myc expression.