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Reassessment of Antigenic Determinant of Saccharomyces cerevisiae Serotype Ia
Author(s) -
Nishikawa Akemi,
Serine Takeshi,
Ikeda Reiko,
Shinoda Takako,
Fukazawa Yoshimura
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01061.x
Subject(s) - mannan , saccharomyces cerevisiae , biology , cryptococcus neoformans , serotype , antigen , microbiology and biotechnology , yeast , mannose , agglutination (biology) , biochemistry , polysaccharide , immunology
We examined the immunochemical structure of the antigenic determinant of S. cerevisiae serotype Ia. The specific factor serum for S. cerevisiae serotype Ia was obtained either from factor 18 serum by adsorption with heat‐killed cells of Candida glabrata , or from anti‐S. cerevisiae Ia (M 6001) serum by adsorption with heat‐killed cells of S. cerevisiae Ib (IFO 0751). We designated this adsorbed serum as factor 18a. Acetolysis of S. cerevisiae cell wall D‐mannan gave five oligosaccharides. Signals of 1 H‐nuclear magnetic resonance spectra of mannooligosaccharides derived from S. cerevisiae mannan were assigned for their linkages by the aid of those of α‐1,3′‐linked mannooligosaccharides derived from glucuronoxylomannan of capsule of Cryptococcus neoformans serotype A‐D. Agglutination‐inhibition experiments revealed that the mannopentaose from S. cerevisiae mannan was the most effective inhibitor. Moreover, inhibitory activities of α‐1,3′‐linked mannotriose, mannotetraose, and mannopentaose which were derived from glucuronoxylomannan of C. neoformans were shown to be higher than those of mannotetraose with one terminal α‐(l‐3) linkage from homologous S. cerevisiae mannan. These results indicate that mannopentaose with terminal two α‐(l‐3) linkages is responsible for the specificity of S. cerevisiae Ia.