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Idiotypical Identity of IgG Myeloma Protein with Monoclonal IgM, Bence Jones Protein, and Fv Derived from One Patient
Author(s) -
Miyagawa Nobuyuki,
Morita Keiko,
Azuma Takachika
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01056.x
Subject(s) - bence jones protein , idiotype , paraproteins , microbiology and biotechnology , clone (java method) , isotype , immunoglobulin idiotypes , immunoglobulin light chain , myeloma protein , biology , monoclonal , antibody , monoclonal antibody , waldenstrom macroglobulinemia , immunology , biochemistry , gene , lymphoma
Serum from a patient (KK) with IgG 2 ‐λ myeloma was shown to contain multiple paraproteins corresponding to an IgM‐λ monoclonal protein (MMP), a λ‐type Bence Jones protein (BJP), and a 30 kDa component in addition to the IgG 2 myeloma protein (GMP). These proteins possessed common idiotypic determinants, as judged by their monoclonal reactivity with rabbit anti‐GMP idiotype antibody (aId) in the immunofixation electrophoresis. Analysis with aId absorbed with either H or L chain of GMP revealed that the 30 kDa component shared both V H and V L with GMP and MMP. while BJP carried only the V L idiotype. The 30 kDa component, however, failed to react with antibody to either the μ, γ, α, K, or λ isotype, indicating that it had an Fv‐like molecular composition. These results suggest that myeloma cells of KK had diverged from the same precursor B cell clone to produce MPs of different isotypes and altered molecular constructions.