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Early Development of CD8‐Negative and CD8‐Positive MHC‐Unrestricted Cytotoxic Cells Induced by Alloantigen Inoculation in Mice
Author(s) -
Nishi Yasuaki,
Hosokawa Tomohide,
Aoike Akira,
Kawai Keiichi,
Kamahora Takeshi
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01055.x
Subject(s) - cytotoxic t cell , spleen , cd8 , biology , antibody , immunology , immune system , antigen , in vitro , biochemistry
Peritoneal cells (PC) in C57B1/6 (B6, H‐2 b ) mice receiving an intraperitoneal (i.p.) injection of allogeneic BALB/c (H‐2 d ) spleen cells demonstrated potent cytotoxic activity against syngeneic, xenogeneic, third‐party allogeneic tumors as well as H‐2 d derived tumors. Maximum cytotoxic activity against various tumors other than H‐2 d derived tumor, B16 (H‐2 b ) was elicited on day 3 post allosensitization and decreased drastically thereafter, whereas cytotoxic activity against P815 (H‐2 d ) peaked 3 days after the inoculation and maintained the peak activity thereafter. Surface phenotype of PC responsible for the cytotoxic activity against B16 was Thy‐1+/‐, Lyt‐2‐, L3T4 − , asialo GM1 (AGM1)+, and that of PC against P815 was Thy‐1+, Lyt‐2+ (or Lyt‐2+/‐), L3T4~, AGM1+. These phenotypes showed similar phenotypes to the counterparts against B16 and against P815 in spleen cells induced by intravenous inoculation of alloantigen. When mice were pretreated i.p . with anti‐AGM1 antibody before the allosensitization, anti‐P815 cytotoxic activity in PC was completely diminished. Similar activity in spleen, however, was enhanced by i.v . treatment with the antibody before the i.v . inoculation of alloantigen. The data clearly demonstrate that in vivo inoculation of B6 mice with normal allogeneic cells induces “NK‐like” CD8‐ cytotoxic cells and “anomalous” CD8 + cytotoxic cells in PC.