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Drug Resistance and R Plasmids in Pasteurella multocida Isolates from Swine
Author(s) -
Yamamoto Junya,
Sakano Tetsuya,
Shimizu Mikio
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01049.x
Subject(s) - pasteurella multocida , biology , plasmid , kanamycin , streptomycin , chloramphenicol , microbiology and biotechnology , ecori , pasteurella , tetracycline , ampicillin , virology , antibiotics , bacteria , dna , genetics
A total of 163 strains of Pasteurella multocida isolated from swine were examined for drug resistance and R plasmids. Strains resistant to sulfadimethoxine (Sa r ), ampicillin (Ap r ), streptomycin (Sm r ), kanamycin (Km r ), and chloramphenicol (Cp r ) were found in 93.9, 1.8, 16.6, 1.2, and 10.4%, respectively. There were two patterns of drug resistance (Sa r and Sa r Cp r ) in isolates from nasal cavities, and five patterns (Sa r , Sa r Sm r , Sa r Sm r Cp r , Sa r Sm r Ap r , and Sa r Sm r Km r Cp r ) in isolates from pneumonic lung specimens. Two isolates studied were proved to carry a nonconjugative R plasmid pJY2 or pJY8 with other unidentified plasmids, respectively. pJY2 (3.6 megadaltons) encoding resistance to Sa r Sm r had one cleavage site for Eco RI or Hind III endonuclease and two sites for Pst I endonuclease. pJY8 (5.5 megadaltons) encoding resistance to Sa r Sm r Km r Cp r had one Eco RI site and two Pst I sites.