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Detection of Common Flagella Antigen in Bacillus cereus by Monoclonal Antibody
Author(s) -
Mikami Takeshi,
Hiraoka Kenji,
Murakami Tomonori,
BoonLong Jotica,
Matsumoto Tatsuji,
Suzuki Masuko
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01048.x
Subject(s) - bacillus cereus , flagellum , biology , antigen , monoclonal antibody , cereus , microbiology and biotechnology , escherichia coli , bacillus subtilis , agglutination (biology) , bacillus (shape) , cross reactivity , epitope , serotype , antibody , cross reactions , bacteria , biochemistry , genetics , gene
Bacillus cereus has been classified into 23 types by immunochemical analyses of flagella antigen, but a common antigenic determinant of flagella had not been determined. When the immunochemical method of classification had changed from “agglutination method” to “enzyme‐linked immunosorbent assay (ELISA),” the cross‐reactivity between each flagella type was increased. These results indicated that the application of ELISA method would enable detection of the common antigenic determinant of B. cereus . Therefore, we attempted to make monoclonal antibody against common flagella antigen that could be detected by ELISA. Monoclonal antibody provided was specific for B. cereus (H‐1 to H‐23) and did not show the cross‐reactivity with Escherichia coli NIH and Bacillus subtilis .