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Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus , and Vibrio cholerae Non O‐1 with Plasmid DNA by Electroporation
Author(s) -
Hamashima Hajime,
Nakano Toshikazu,
Tamura Shinobu,
Arai Taketoshi
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01047.x
Subject(s) - plasmid , vibrio alginolyticus , electroporation , biology , transformation (genetics) , microbiology and biotechnology , vibrio cholerae , vibrio parahaemolyticus , vibrio , vibrionaceae , dna , genetics , gene , bacteria
An electroporation procedure for the plasmid‐mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus , and V. cholerae non O‐1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug‐resistance genes on the vector plasmid were expressed in the Vibrio strains.