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Serotype Analysis of Group A Human Rotavirus Related to Acute Gastroenteritis in Winter in Gifu City
Author(s) -
Kawamoto Hiroyoshi,
Tanaka Hiroshi,
Urasawa Shozo,
Urasawa Tomoko,
Taniguchi Koki
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01043.x
Subject(s) - serotype , rotavirus , feces , biology , virology , acute gastroenteritis , reoviridae , veterinary medicine , microbiology and biotechnology , virus , medicine
A total of 348 fecal specimens collected from children with acute gastroenteritis in Gifu city over three consecutive winter seasons (from November 1986 to March 1989) were examined for group A human rotavirus (HRV) by either a commercial test kit or a sandwich enzyme‐linked immunosorbent assay (sandwich ELISA). One hundred twelve of the 173 group A HRV‐positive specimens were further subjected to serotype determination by ELISA with four serotype‐specific monoclonal antibodies to VP7 (ELISA‐serotyping). Ninety‐one specimens (81.3%) were successfully serotyped: 41 (36.6%) were serotype 1, 13 (11.6%) serotype 2, 27 (24.1%) serotype 3, and 10 (8.9%) serotype 4. The serotypes of the remaining 21 (18.8° 0 ) could not be determined. The predominant serotype of HRV that prevailed in Gifu city changed every winter: serotype 3 (63.4%) was most prevalent in the 1st winter, serotype 4 (42.9%) in the 2nd winter, and serotype 1 (64%) in the 3rd winter.