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Localization of a Determinant Mediating Partial Macrolide Resistance in Staphylococcus aureus
Author(s) -
Matsuoka Mayumi,
Endou Kikutarou,
Nakajima Yoshinori
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01040.x
Subject(s) - ethidium bromide , staphylococcus aureus , biology , microbiology and biotechnology , tylosin , macrolide antibiotics , antibiotics , 23s ribosomal rna , bacteria , genetics , erythromycin , dna , gene , rna , ribosome
Four out of more than 8,200 Staphylococcus aureus strains isolated in Japan between 1961 and 1980 were constitutively resistant to a variety of macrolide antibiotics except tylosin and rokitamycin, but susceptible to lincosamide and streptogramin type B antibiotics (PM). The data obtained by agarose gel electrophoresis, CsCl‐ethidium bromide density gradient analysis, diagnosis with ATP‐dependent deoxyribonuclease, and a test transducing into a rec − mutant with phage 80L2 propagated on PM‐resistant S. aureus all suggested that the determinant for the PM‐resistance is located in chromosome.