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Rapid Small‐Scale Preparation Method of Cell Surface Polysaccharides
Author(s) -
Sugiyama Tsuyoshi,
Kido Nobuo,
Arakawa Yoshichika,
Mori Masashi,
Naito Setsuko,
Ohta Michio,
Kato Nobuo
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01039.x
Subject(s) - ouchterlony double immunodiffusion , polysaccharide , chromatography , biology , gel electrophoresis , nitrocellulose , serotype , immunodiffusion , mannan , microbiology and biotechnology , polyacrylamide gel electrophoresis , lipopolysaccharide , antigen , blot , sodium dodecyl sulfate , biochemistry , chemistry , membrane , immunology , gene , enzyme , antiserum
A rapid small‐scale method of extraction of lipopolysaccharide (LPS) and capsular polysaccharides was developed for the purpose of identification of chemotypes of LPS and serotypes of capsular antigens. Cell surface polysaccharides were prepared within less than 2 hr from 1.5 ml of broth or suspension of colonies cultured overnight. The preparations were analyzed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis for LPS, and by double diffusiori gel precipitation (Ouchterlony) test and blotting to nitrocellulose membrane for capsular polysaccharide. The analyses with the preparations obtained by the method could provide adequate results capable of identifying chemotypes of LPS and serotypes of capsular antigens.