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Electron Microscope Studies on the Intracellular Growth of PL‐1 Phage of Lactobacillus casei
Author(s) -
Watanabe Kenji,
Fukuzaki Tomoharu,
Shirabe Mitsuhiro,
Nakashima Yukihiko,
Murata Kunihiko,
Kuroiwa Ataru
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01030.x
Subject(s) - lactobacillus casei , biology , cytoplasm , electron microscope , intracellular , lactobacillus , bacteriophage , microbiology and biotechnology , biophysics , bacteria , biochemistry , genetics , physics , optics , escherichia coli , gene
Ultrathin sections of the cells of Lactobacillus casei infected with or without PL‐1 phages were observed by the rapid‐freezing and substitution‐fixation method. Phage‐head‐like particles were first observed in the nuclear region. The region was seen more widely dispersed in the cytoplasm than that observed by the conventional chemical fixation method. The features of cells just broken open by the infected phages were observed by the sedimentation method devised by us. The bursting occurred in more than one place in the cells with liberation of progeny phages.