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Demonstration of RNA N ‐Glycosidase Activity of a Vero Toxin (VT2 Variant) Produced by Escherichia coli 091: H21 from a Patient with the Hemolytic Uremic Syndrome
Author(s) -
Furutani Masayuki,
Ito Kiyoshi,
Oku Yuichi,
Takeda Yoshifumi,
Igarashi Kazuei
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01019.x
Subject(s) - biology , ribosome , escherichia coli , vero cell , ribosome inactivating protein , rna , toxin , ribosomal rna , microbiology and biotechnology , glycoside hydrolase , 50s , biochemistry , enzyme , in vitro , gene
A new Vero toxin purified from Escherichia coli O91: H21 isolated from a patient with the hemolytic uremic syndrome (VT2vh) was shown to inhibit elongation factor 1‐dependent aminoacyl‐tRNA binding to ribosomes, resulting in inhibition of protein synthesis in rabbit reticulocytes. VT2vh, like Shiga toxin, VT1 and VT2, showed RNA N ‐glycosidase activity and cleaved the N ‐glycosidic bond of the adenosine residue at position 4324 in 28S ribosomal RNA.