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Comparative Evaluation on Mouse Nasal Immunogenicity of Arylmethane‐, Xanthene‐, Quinone‐Imine‐, and Acridine‐Dye‐Inactivated Sendai Virus Vaccines
Author(s) -
Miyamae Takeo
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01017.x
Subject(s) - immunogenicity , malachite green , microbiology and biotechnology , methylene blue , titer , chemistry , biology , virology , virus , biochemistry , antibody , immunology , organic chemistry , adsorption , photocatalysis , catalysis
Twenty‐seven kinds of organic dye‐inactivated Sendai virus vaccines were prepared by treatment in dark at 23 C for 2 months or more, and selected with the high HA titers as a guide. Their nasal immunogenicities were examined in mice by contact infection and immunofluorescent method, and the relative merits of the dye‐inactivants were determined. The strongest protection was elicited with acriflavine‐. auramine O‐, eosin Y‐, neutral red‐, night blue‐, patent blue V‐, thymol blue‐. uranin‐, and xylene cyanol FF‐treated vaccines. Middling protective efficacy was induced by use of erio green B‐, malachite green‐, methyl green‐, proflavine‐, pyronin B‐, and thionin‐inactivated vaccines. Dye‐inactivated vaccines that resulted in the weakest protection were Bindschedler's green‐, bromothymol blue‐, erythrosin B‐, ethyl violet‐, gallein‐, light green SF yellowish‐, methyl violet‐, new methylene blue N‐, phenol red‐, rhodamine 6G‐, spirit blue‐ and victoria blue B‐treated ones. Serum HI titers developed by nasal vaccination were variable, and rose still more in most vaccinated groups postexposure. Elicitation of the most effective nasal immunogenicity in dye‐inactivated vaccines appeared to depend on selective modification of capsid protein or ribose in viral core with dyes possessing definite functions, despite the different molecular structures.