Premium
Chemiluminescence Response of Human Phagocytes against IgG‐Opsonized Staphylococci with and without Protein A Activities
Author(s) -
Yamada Sakuo
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb01005.x
Subject(s) - antibody opsonization , opsonin , protein a , biology , staphylococcus aureus , immunoglobulin g , phagocytosis , chemiluminescence , antibody , microbiology and biotechnology , phagocyte , immunology , bacteria , chemistry , genetics , organic chemistry
The effects of immunological IgG binding to Staphylococcus aureus and IgG binding via protein A on the chemiluminescence (CL) response of human phagocytes were examined. The results obtained by enzyme immunoassay showed a clear correlation between the magnitude of the CL response and amount of IgG on protein A‐deficient HL‐87 strain. Despite no difference in protein A activity between 209P and Cowan I strains, the CL response to IgG‐opsonized 209P cells was lower than that to Cowan I cells similarly opsonized. Moreover, the CL response to opsonized HL‐87 cells was identical with that of opsonized Cowan I cells, which was a protein A‐rich parent strain of the HL‐87. The protein A activity of Cowan I cells was significantly decreased when the cells were treated with the Fc fraction of IgG before opsonization, but such a treatment did not change the phagocytic CL response. These results strongly suggest that IgG bound to protein A via its Fc portion has no effect on the phagocytic CL response and that IgG immunologically bound to S. aureus is responsible for the opsonization of the bacteria.