Premium
Immunological Characterization of Lipopolysaccharides from Proteus Strains Used in Weil‐Felix Test and Reactivity with Patient Sera of Tsutsugamushi Diseases
Author(s) -
Amano KenIchi,
Mizushiri Sakae,
Fujii Shunsaku,
Fukushi Kazue,
Suto Tsunehisa
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb00998.x
Subject(s) - antiserum , proteus , microbiology and biotechnology , titer , biology , antigen , rickettsia , rickettsia typhi , heterologous , lipopolysaccharide , virology , antibody , escherichia coli , immunology , virus , biochemistry , gene
Immunological analyses of lipopolysaccharides (LPS) isolated from Proteus strains OX2, OX19, and OXK used as antigens of Weil‐Felix (WF) test, were performed by quantitative agglutination, enzyme‐linked immunosorbent assay (ELISA), and immunoblotting. Antisera against LPS and whole cells (WC) of the three Proteus strains reacted with homologous LPS but not with heterologous LPS. and the reaction was inhibited by the O‐polysaccharide fraction isolated from the homologous LPS except OX19‐LPS. which lacked O‐polysaccharide moiety. The immunological data support the findings that the O‐polysaccharide moieties of LPS from OX2 and OXK strains possess different chemical composition (Mizushiri, Amano, Fujii, Fukushi, and Watanabe, Microbiol. Immunol. 34: 121 133, 1990). Antisera against Proteus strains reacted weakly with WC of Rickettsia prowazekii, Rickettsia typhi , and Rickettsia tsutsugamushi . Antisera from patients with tsutsugamushi disease reacted with OXK‐WC by WF test when the sera were obtained 13 days after onset of fever. The immunoperoxidase (IP) test titers of these antisera began to rise 6 days after the onset of fever. By ELISA tests these antisera reacted with OXK‐WC and OXK‐LPS independently of the titers of WF or IP tests.