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Cell Surface of a Tetrads‐Forming Mutant of Micrococcus luteus : Chemical Treatment of the Cells and Teichuronic Acids on the Surface
Author(s) -
Monodane Toshihiko,
Tokunaga Michiko,
Torii Mitsuo
Publication year - 1990
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1990.tb00992.x
Subject(s) - micrococcus luteus , antiserum , mutant , biology , tetrad , micrococcus , microbiology and biotechnology , reagent , biochemistry , bacteria , antibody , chemistry , escherichia coli , botany , genetics , immunology , gene
Tetrads‐forming mutant MT cells of Micrococcus luteus , both treated with chemical reagents and non‐treated, were observed with a scanning electron microscope (SEM). The agglutinability of the cells with antiserum containing anti‐teichuronic acid antibody was examined. The binding of protein A‐gold particles to the cells, mediated with the antiserum, was also observed with SEM. A tetrad surface, not surface of each of four “unit monococci” constituting a tetrad, consisted of two or three smooth areas with borders. The difference in the surface features between M. luteus wild‐type IFO 3333 (Monodane et al, Microbiol. Immunol. 33: 165–174, 1989) and the mutant MT cells is discussed.