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Characteristics of Macrophage Activation by Gamma Interferon for Tumor Cytotoxicity in Peritoneal Macrophages and Macrophage Cell Line J774.1
Author(s) -
Miyakawa Yozo,
Kagaya Keiko,
Watanabe Koji,
Fukazawa Yoshimura
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb03160.x
Subject(s) - macrophage , cytotoxicity , biology , cell culture , interferon gamma , immunology , cytokine , in vitro , biochemistry , genetics
We investigated the characteristics of macrophage‐mediated tumor cytotoxicity (MTC) against Meth A target, H 2 O 2 generation and release of effector molecule(s) for MTC, by comparing with those of peritoneal macrophages (PMP) and macrophage cell line J774.1 during stimulation with recombinant gamma interferon (IFN‐γ). In PMP, MTC was demonstrated when they were stimulated with IFN‐γ for 12 hr (short‐term stimulation) and was abrogated when they were stimulated for 48 hr (long‐term stimulation). Enhanced H 2 O 2 generation was observed in PMP activated by long‐term stimulation followed by triggering with PMA, but not observed by triggering with Meth A cells. By contrast, whereas non‐treated J774.1 cells have already attained a definite level of MTC, a higher MTC level was demonstrated both by short‐ and long‐term stimulations. Conversely, J774.1 cells were unable to generate H 2 O 2 at any stage of IFN‐γ stimulation followed by triggering both with PMA or Meth A cells. The time course for stimulation of PMP by IFN‐γ for release of cytotoxic factor (CF) corresponded to that for MTC by PMP, and activities of the CF released from both activated PMP and J774.1 cells also closely corresponded to those of MTC by both cells. The serological and physicochemical characteristics of CF released from both activated PMP and J774.1 cells were determined to be closely related to those of tumor necrosis factor (TNF). These results indicate that in contrast to PMP, the J774.1 cell line is free from suppression stage for MTC and CF release during stimulation with IFN‐γ. The results suggest that TNF‐like CF plays a crucial role for MTC against Meth A target, and that H 2 O 2 is irrelevant for MTC against Meth A.