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Spreading of Streptomycin Antibiotic Resistance Gene in Escherichia coli Plasmids Demonstrated by Southern Blot Analysis
Author(s) -
Hajnal Anna,
Falus Andras
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01993.x
Subject(s) - plasmid , escherichia coli , streptomycin , biology , southern blot , gene , aminoglycoside , microbiology and biotechnology , genetics , antibiotics
Plasmids from E. coli strains of 38 donors were transconjugated to common recipient SY663 Escherichia coli K12. The restriction patterns of the isolated plasmids were highly heterogenous. However, the streptomycin (Sm) resistance genes of the plasmids were identical or closely homologous in 29 of the 33 plasmids conferring Sm resistance. These data were based on Southern blot analysis, using the Sm resistance gene (encoding aminoglycoside phosphoryl transferase) as probe cut out from pBPl plasmid. Our data suggest an extensive spreading of streptomycin resistance gene of this type.