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Bindings of Toxic Shock Syndrome Toxin‐1 and Staphylococcal Enterotoxins A, B, and C to Rabbit Spleen Cells
Author(s) -
Fujikawa Hiroshi,
Takayama Hiroo,
Uchiyama Takehiko,
Igarashi Hideo
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01986.x
Subject(s) - toxic shock syndrome , enterotoxin , toxin , spleen , superantigen , biology , binding site , dissociation constant , microbiology and biotechnology , staphylococcus aureus , receptor , biochemistry , immunology , bacteria , escherichia coli , genetics , gene
Toxic shock syndrome toxin‐1 (TSST‐1) and staphylococcal enterotoxins (SE) A, B, and C were studied on binding to rabbit spleen cells. The toxins showed remarkable mitogenic effects on the cells. Among them, SEA and TSST‐1 had much stronger mitogenic activities than SEB and SEC. Binding study showed that labeled TSST‐1 and SEA bound considerably to cells, but that labeled SEB or SEC was not observed to bind at a detectable level under the same conditions as TSST‐1 and SEA. Competitive binding analysis between toxins to cells proved that TSST‐1 and SEA clearly competed with each other in binding. Scatchard plots for TSST‐1 and SEA in binding were linear at the doses used. The Scatchard analysis for TSST‐1 and SEA gave a dissociation constant of 2.5 × 10 −9 m and 7.6 × 10 −8 m and the number of binding sites per cell of 5.3 × 10 3 and 1.0 × 10 5 , respectively.