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Characterization of Activation of a Partially Defective B Cell Subpopulation with Immunocompetence Restricted to IgM and IgA
Author(s) -
Nakaniwa Hiroshi,
Kajikawa Taketsugu,
Bito Yukio
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01517.x
Subject(s) - biology , activator (genetics) , immunocompetence , intracellular , microbiology and biotechnology , b cell , protein kinase c , antigen , calcium , b 1 cell , immunology , t cell , antibody , antigen presenting cell , kinase , medicine , biochemistry , receptor , immune system
A B‐cell subpopulation (B M‐A cell) responding to an antigen with the production of IgM and IgA plaque‐forming cells but not of IgG plaque‐forming cells was isolated from neonatally bursectomized chickens and was examined for the mode of activation by B‐cell mitogens. The B M‐A cells did not elevate both glucose consumption and protein synthesis with the B‐cell mitogens, in striking contrast to normal B cells. The B M‐A cells were also not activated by an activator of protein kinase C, phorbol myristate acetate. Both anti‐Ig and a calcium ionophore, A23187, however, primed the B M‐A cells to increase intracellular free calcium ion as well as normal B cells. From these results it is conceived that the lack of protein kinase C activation may be responsible for the failure of activation of B M‐A cells.