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Isolation of Line 10 Hepatoma‐Cell Membrane by the Water Extraction Method and Immunochemical Analysis
Author(s) -
Ahsan Chowdhury Rafiqul,
Sasaki Jinichi
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01515.x
Subject(s) - glycoprotein , immunoelectrophoresis , biology , cell culture , molecular mass , microbiology and biotechnology , strain (injury) , membrane glycoproteins , guinea pig , biochemistry , chromatography , antigen , chemistry , immunology , enzyme , anatomy , genetics
Water extraction method was applied to isolate the cell membrane from line 10 hepatoma cells and normal liver cells in strain 2 guinea pig. The materials isolated by this method were further analyzed by different immunochemical techniques including SDS‐PAGE, crossed immunoelectrophoresis and crossed affino immunoelectrophoresis to demonstrate the major components and their antigenicities. Five major glycoproteins of apparent molecular weights of 44, 46, 62, 64, and 68 kDa were prominent in line 10 tumor cell materials, whereas one band of molecular weight of 82 kDa was prominent in the materials from normal liver cells. Also four minor components from line 10 tumor cells were found to be glycoprotein in nature.