Premium
A Non‐Radioactive DNA Probe for the Detection of Mycoplasma pulmonis in Murine Mycoplasmosis
Author(s) -
Matsuzaki Michiya,
Harasawa Ryô,
Kimizuka Fusao,
Koshimizu Kaoru
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01505.x
Subject(s) - biology , mycoplasma , hybridization probe , mycoplasmataceae , dna , mollicutes , microbiology and biotechnology , molecular probe , nucleic acid thermodynamics , virology , in situ hybridization , genetics , base sequence , gene , gene expression
A non‐radioactive DNA probe for the detection of Mycoplasma pulmonis was developed by using photobiotin. The probe detected 0.3 ng chromosomal DNA of M. pulmonis. No cross‐hybridization was observed between the probe and the other murine mycoplasma species, M. arthritidis and M. neurolyticum.