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Location on the Chromosome of the lac Gene in a Lactose‐Fermenting Salmonella litchfield Strain
Author(s) -
Hamada Kokichi,
Nakayama Yasunori
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01501.x
Subject(s) - biology , lactose , salmonella , genetics , lac operon , gene , chromosome , strain (injury) , escherichia coli , microbiology and biotechnology , bacteria , biochemistry , anatomy
We present conclusive evidence for the chromosomal location of the lac gene in a lactose‐fermenting Salmonella litchfield strain (AO Lac + ). Two Hfr strains constructed from AO Lac + had abilities to transfer the lac gene to S. typhimurium LT2 at relatively high frequencies. Detailed characterization of the transconjugants suggested that the lac in AO Lac + was located on the host chromosome between galE (18 min) and trpB (34 min). Transduction experiments using P22 phage showed that the lac was cotransduced with gal , but not with trpB. These results clearly indicate that the lac gene is located at a position near 18 min of the linkage map of Salmonella.