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A Simple Method for Observation of Phagocytosis on Bacterial Thin‐Layer
Author(s) -
Seki Keiko,
Murai Miyo,
Sakurada Junji,
Shirahige Akira,
Kobayashi Noriyuki,
Hwang Soomyung,
Masuda Shogo
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01500.x
Subject(s) - phagocytosis , opsonin , staphylococcus aureus , magnification , biology , microbiology and biotechnology , whole blood , layer (electronics) , immunology , bacteria , materials science , optics , nanotechnology , genetics , physics
A simple method was developed to visually present the phagocytic activity of leukocytes by using adherent Staphylococcus aureus cells and blood applied on a plastic dish. When heparinized blood was applied on thin‐layer of heat‐killed S. aureus cells on the plastic dish, plaques due to the phagocytic activity of leukocytes were observed with a microscope under a low magnification. Fewer and smaller plaques were observed when plasma‐deprived rather than whole blood was used. Some analyses were made in respect to the fundamental conditions required for optimal results. This method was considered to be useful for conveniently evaluating the serum opsonin activities and phagocytic function of leukocytes in various kinds of diseases.