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Pili of Vibrio cholerae O1 Biotype El Tor: A Comparative Study on Adhesive and Non‐Adhesive Strains
Author(s) -
Iwanaga Masaaki,
Nakasone Noboru,
Ehara Masahiko
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb01492.x
Subject(s) - pilus , pilin , vibrio cholerae , el tor , biology , microbiology and biotechnology , gel electrophoresis , protein subunit , polyacrylamide gel electrophoresis , adhesive , sodium dodecyl sulfate , fimbria , colonization , vibrionaceae , escherichia coli , bacteria , biochemistry , chemistry , enzyme , genetics , gene , organic chemistry , layer (electronics)
Pili were found on the cell surface of non‐adhesive Vibrio cholerae O1 Biotype El Tor as well as the adhesive strain. Purified pili of the adhesive and non‐adhesive strains were morphologically, electrophoretically, and immunologically, indistinguishable from each other. The molecular weights of both pilin (subunit protein of the pilus) were about 16,000 daltons as determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. These 16 kDa pili are different from the pilus colonization factor, which is a 20.5 kDa protein, reported by Taylor et al. The 16 kDa pili of Vibrio cholerae O1 Biotype El Tor have hemagglutinating activity, but may have no role in colonization, because non‐adhesive strains also have such pili.