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In Situ Infiltration of Natural Killer‐Like Cells Induced by Intradermal Injection of the Nucleic Acid Fraction from BCG
Author(s) -
Kuramoto Etsuro,
Toizumi ShinIchi,
Shimada Shizuo,
Tokunaga Tohru
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb00980.x
Subject(s) - nucleic acid , biology , infiltration (hvac) , immunofluorescence , microbiology and biotechnology , peroxidase , peripheral blood mononuclear cell , in vitro , antibody , immunology , enzyme , biochemistry , physics , thermodynamics
Intradermal injection of MY‐1, a nucleic acid fraction extracted from Mycobacterium bovis strain BCG, induced in situ infiltration of mononuclear cells, most of which were asialo GM1 (GA1)‐positive as determined by immunofluorescence microscopy. The infiltration occurred with as little as 1 μ g of MY‐1 and lasted for a week. Double immunofluorescence microscopy revealed that the infiltrating GA1‐positive cells were all positive for Ly‐5, and partially positive for Thy‐1.2, but negative for Mac‐1, Ia, μ ‐chain, Lyt‐1, Lyt‐2, L3T4, and Fc receptor II. They contained neither peroxidase nor nonspecific esterase. The infiltrating cells thus markedly resembled natural killer (NK) cells in their cytochemical characteristics and surface markers. DNase and RNase destroyed the GA1‐positive cell‐inducing activity of MY‐1. These results indicate that the nucleic acid components of MY‐1 are responsible for this effect.