Premium
Monoclonal Antibodies against the Surface Antigens of Shigella flexneri Serotype 1b and Shigella dysenteriae Serotype 1
Author(s) -
Suzuki Kumiko,
Takeda Tae
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb00977.x
Subject(s) - shigella flexneri , shigella dysenteriae , monoclonal antibody , microbiology and biotechnology , biology , serotype , antigen , virology , shigella , agglutination (biology) , antibody , escherichia coli , salmonella , immunology , bacteria , biochemistry , gene , genetics
Monoclonal antibodies against the surface antigens of Shigella flexneri 1b and S. dysenteriae 1 were prepared. The specificities of the antibodies were evaluated by enzyme‐linked immunosorbent assay (ELISA), and quantitative agglutination using microtiter plate. Monoclonal antibodies against S. flexneri 1b, designated Sf2B2 and Sf2G4, belonged to IgG2a and IgG1 subclass, respectively. The former was specific for S. flexneri 1b, whereas the latter was reactive not only to S. flexneri 1b, but also weakly to 3a and 4b. Monoclonal antibody against S. dysenteriae 1, Sd5E1 (IgM), reacted with S. dysenteriae 1, 3, 6, 7, and S. boydii 2.