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Productive Ascites Growth of Heterohybridomas between Epstein‐Barr Virus‐Transformed Human B Cells and Murine P3×63Ag8.653 Myeloma Cells
Author(s) -
Matsumoto Mitsuru,
Mochizuki Kenichi,
Kobayashi Yuzuru
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb00975.x
Subject(s) - biology , ascites , virology , virus , monoclonal antibody , epstein–barr virus , antibody , multiple myeloma , myeloma protein , microbiology and biotechnology , ascitic fluid , immunology , medicine
Heterohybridomas between Epstein‐Barr virus‐transformed human B cells and murine myeloma P3×63Ag8.653 cells were found to produce sizable quantities of human monoclonal antibodies in nude mouse ascites. Mice injected intraperitoneally with two of such heterohybridomas yielded several milliliters of ascites fluid which contained nearly 100‐fold higher anti‐platelet activities than those in routine culture supernatants.