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The Effect of GD 3 Ganglioside Obtained from Bovine Lymphosarcoma on Bovine Normal Mononuclear Cell
Author(s) -
Yasutomi Yasuhiro,
Onuma Misao,
Watarai Shinobu,
Yasuda Tatsuji,
Kirisawa Rikio,
Kawakami Yoshimi
Publication year - 1989
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1989.tb00971.x
Subject(s) - microgram , concanavalin a , peripheral blood mononuclear cell , biology , microbiology and biotechnology , lymphocyte , immunology , mixed lymphocyte reaction , cytotoxic t cell , cell culture , ganglioside , in vitro , t cell , immune system , biochemistry , genetics
The effect of immunological function of GD 3 on normal bovine lymphocyte was examined with various in vitro assay systems. The GD 3 level in sera from enzootic bovine leukemia (EBL) cattle was significantly increased compared with that of normal cattle (EBL: 0.62±0.24 μ g/ml; normal cattle: 0.33±0.09 μ g/ml, P <0.05). Lymphocyte blastogenesis elicited by concanavalin A was inhibited by addition of a 50 μ g/ml concentration, or more, of GD 3 . Inhibitory effect of GD 3 in IL‐2‐dependent T cell line and EBL tumor cell line was hardly observed compared with normal peripheral blood mononuclear cells. GD 3 also inhibited mixed lymphocyte reaction and allo cytotoxic T lymphocyte reaction.