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Adsorption of LLCMK 2 Cell‐Grown Sendai Virus onto Human Red Blood Cells and Its Release from the Virus Adsorbed Cells
Author(s) -
Komatsu Hiroyoshi,
Tozawa Hideki
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01484.x
Subject(s) - sendai virus , virus , biology , neuraminidase , incubation , hemagglutinin (influenza) , adsorption , virology , clostridium perfringens , red blood cell , microbiology and biotechnology , biochemistry , chemistry , bacteria , genetics , organic chemistry
An early stage of virus adsorption was studied in a system of Sendai virus metabolically labeled with [ 3 H] leucine in LLCMK 2 cells and of human red blood cells (RBCs). The efficiency of viral release from the virus‐bound RBCs by incubation at 37 C depended on the number of virus particles which had been used for adsorption onto the RBC at 4 C. When 7.8 × 10 2 virus particles were previously adsorbed onto the RBC at 4 C, most of the viruses were dissociated from the RBC at 37 C. In the case of adsorption of 3 to 12 virus particles per RBC, however, most of the viruses were not dissociated from the RBC by incubation at 37 C. Such RBC‐bound viruses were released by incubation with various bacterial neuraminidases ( Clostridium perfringens , etc.) or with a large number of LLCMK 2 cell‐grown Sendai virus (LLCMK 2 ‐Sendai) particles, but not released by treatment with hemagglutinin‐neuraminidase protein (Sendai‐gp) isolated from egg‐grown Sendai virus.