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New Complement Fixation Test with Peroxidase‐Labeled Complement Clq for Direct and Quantitative Determination of Antibodies to Herpes Simplex Virus
Author(s) -
Taguchi Fumiaki
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01480.x
Subject(s) - complement fixation test , herpes simplex virus , antibody , virology , antigen , peroxidase , virus , biology , microbiology and biotechnology , enzyme , immunology , serology , biochemistry
An enzyme‐labeled complement fixation (ELISA‐CF) test for the direct and quantitative determination of complement fixing (CF) antibodies has been developed. This paper described the introduction of the ELISA‐CF test that used peroxidase‐labeled Clq component of complement to detect CF antibodies which had reacted with herpes simplex virus (HSV), as a virus model. Equal volumes of heat‐inactivated serum and the peroxidase‐labeled Clq (P*‐Clq) were simultaneously added to wells of microplates which had been coated with HSV CF antigen or with cell control antigen. The enzymatic activities of P*‐Clq bound to the immune complex were determined photometrically. The ELISA‐CF test allows processing of serum specimens in a 3‐hr operation, with procedural simplicity and increased specificity and sensitivity compared with the conventional CF test.