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Method of Genomic DNA Cloning by the Combination of Cosmid Shuttle Vector and Monoclonal Antibody
Author(s) -
Tagawa Masatoshi,
Ito Toshihiro,
Kanno Masamoto,
Okitsu Akira,
Sakamoto Tohru,
Imai Kenji,
Kuwabara Ichiro,
Koseki Haruhiko,
Matsubara Hisahiro,
Lau Yunfai,
Taniguchi Masaru
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01471.x
Subject(s) - cosmid , biology , monoclonal antibody , shuttle vector , genomic dna , microbiology and biotechnology , genomic library , cloning (programming) , transfection , antigen , multiple cloning site , molecular cloning , dna , antibody , virology , vector (molecular biology) , recombinant dna , genetics , cell culture , complementary dna , gene , base sequence , computer science , programming language
We report here the strategy to isolate the DNA fragment of any species origin which encodes cell surface antigen by using cosmid library transfection and cell sortings with a monoclonal antibody. We took the mouse melanoma antigen defined by monoclonal antibody as a model system and rescued the genomic DNA by in vitro packaging, showing the feasibility of this procedure.