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Immunoelectron Microscopic Studies on Spore Coat Proteins of Bacillus megaterium
Author(s) -
Imagawa Masayoshi,
Ohtsuka Mikiko,
Nakatani Yoshihiro,
Oku Yuichi,
ElBelbasi Hussein I.,
Nishihara Tsutomu,
Kondo Masaomi
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01463.x
Subject(s) - immunogold labelling , bacillus megaterium , biology , cytoplasm , immunoelectron microscopy , coat , spore , antibody , antiserum , microbiology and biotechnology , biochemistry , bacteria , immunology , paleontology , genetics
An immunochemical staining technique for the spore coat proteins of Bacillus megaterium ATCC 12872 was developed using colloidal gold as a second antibody. For reducing the non‐specific immunogold binding and increasing the specific binding, the affinity‐purified IgG was used as a first antibody. In sporulating cells at t 10 , gold particles were found not only in the spore coat but also in the mother cell cytoplasm, suggesting that some coat proteins were synthesized in the cytoplasm. Use of the specific affinity‐purified antibody to 48K‐protein demonstrated that this protein was one of the components of the outer coat.