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Experimental Chronic Pulmonary Infection in Mice Caused by Klebsiella pneumoniae
Author(s) -
Iizawa Yuji,
Nishi Takeshi,
Kondo Masahiro,
Imada Akira
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01451.x
Subject(s) - klebsiella pneumoniae , lung , biology , respiratory tract , pathogenesis , respiratory system , lesion , respiratory disease , microbiology and biotechnology , chronic infection , pathology , pulmonary infection , immunology , medicine , immune system , anatomy , escherichia coli , biochemistry , gene
Examination of mouse strain differences in susceptibility to experimental respiratory tract infection with Klebsiella pneumoniae 27 revealed that a chronic pulmonary infection model could be established using CBA/J mice. After 6 × 10 5 colony‐forming units of K. pneumoniae 27 were inoculated into the lung, the bacterial counts in the lungs changed with time showing four different phases: initial decrease, regrowth, steady‐state, and final increase leading to death. Throughout the course of the infection, the challenge bacteria were isolated mainly from the respiratory organs. Pulmonary gross lesions appeared on day 2 after infection and persisted thereafter. Lobar consolidation was the primary lesion and occurred mainly in the anterior and middle lobes of the right lung, and the median lobe. Mice began to die from 4 weeks after aerosol exposure. This model may be useful for investigating the pathogenesis of chronic pulmonary infection by Klebsiella and its therapy.