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Restriction Endonuclease DNA Analysis of Leptospira interrogans Serovars Icterohaemorrhagiae and Copenhageni
Author(s) -
Tamai Tomonori,
Sada Eiji,
Kobayashi Yuzuru
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01450.x
Subject(s) - hindiii , restriction enzyme , haeiii , xhoi , ecori , biology , bglii , endonuclease , leptospira interrogans , microbiology and biotechnology , dna , serotype , genetics , psti , leptospira , restriction fragment length polymorphism , genotype , gene
Leptospira interrogans serovar icterohaemorrhagiae strains Ictero No. I and RGA and serovar copenhageni strains M20, Shiromizu and Shibaura were examined by restriction endonuclease DNA analysis. Fifteen endonucleases ( Alu I, Bam HI, Bgl II, Eco RI, Hae III, Hha I, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sty I, Xba I and Xho I) were used as the digesting enzymes. Strain Ictero No. I showed endonuclease cleavage patterns which differed from those of the other four strains only when it was digested with enzymes Kpn I and Hind III. When digested with Kpn I, an extra band of about 5.4 kb was clearly produced, and when digested with Hind III, an extra band of about 25 kb was produced. When the other 13 enzymes were used, no differences were found between the endonuclease cleavage patterns among the five strains. Moreover, strains RGA, M20, Shiromizu and Shibaura could not be distinguished by the restriction endonuclease DNA analysis using all 15 endonucleases. In addition, six newly isolated leptospires from patients with leptospirosis and from Rattus norvegicus were compared with the Ictero No. I and M20 strains, by restriction endonuclease DNA analysis using enzymes Kpn I and Hind III. Three leptospires belonging to serovar icterohaemorrhagiae showed the same endonuclease cleavage patterns as the M20 strain. The other three strains, which belong to serovar copenhageni , showed almost the same endonuclease cleavage patterns as the M20 strain; only the Kaijima 702 strain produced an extra band which was not identical to the Ictero No. I‐specific extra band when digested with Hind III. The leptospiral restriction endonuclease DNA analysis has revealed taxonomic structures that are unrecognized by serology alone.