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Upstream Region of Hepatitis B Virus S Gene Responsible for Transcriptional Stimulation by Dexamethasone
Author(s) -
Masuda Michiaki,
Lee Ganhon,
Yuasa Tazuko,
Yoshikura Hiroshi
Publication year - 1988
Publication title -
microbiology and immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.664
H-Index - 70
eISSN - 1348-0421
pISSN - 0385-5600
DOI - 10.1111/j.1348-0421.1988.tb01435.x
Subject(s) - biology , virology , dexamethasone , stimulation , gene , virus , hepatitis b virus , hepatitis a virus , genetics , endocrinology
Transcriptional regulation of hepatitis B virus (HBV) surface antigen (HBs Ag) gene was studied in human hepatoma‐derived cell lines. Treatment with dexamethasone (Dex; 1 μ M) induced an increase in the smaller HBs‐mRNA initiated within Pre‐S region encoding S and Pre‐S2 proteins, but not the larger HBs‐mRNA initiated in the further upstream encoding Pre‐S1 protein. The Bg1 II‐ Mst II fragment (map position 2425–3201) in the upstream of the S gene was used as a transcriptional promoter of chloramphenicol acetyltransferase (CAT) gene. The CAT activity brought about by this construct in the transient assay was elevated by 5‐fold in the presence of Dex. Deletion analysis localized the sequence required for the full response to Dex within a 590‐base pair fragment in the upstream of the transcriptional initiation site of the smaller HBs‐mRNA. And this fragment contained the binding site for the nuclear factor I (NF‐I), which might have some role in Dex‐dependent transcriptional stimulation.